Composite

Part:BBa_K5311010:Design

Designed by: Carlos Perez de Arenaza and Jose Luis Perez   Group: iGEM24_UPF-Barcelona   (2024-09-26)


MG_010 + RBS cell free MJ + Cry3Aa + RoxP + His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 553
    Illegal BsaI.rc site found at 380


Design Notes

For this composite part we have codon optimized the CDS for the Cry3Aa toxin to be more compatible with C. acnes, we also introduced the standard prefix and sufixes in our sequence to ensure a proper alignment for the Gibson Assembly


Source

This part was created by using the corresponding sequences on this registry: MG10: Part:BBa_J23119; RBS cell free MJ: Part:BBa_K5311004 ; Cry3Aa: Part:BBa_K5311002; RoxP: Part:BBa_K5311001; and the His-tag corresponds to a sequence of 6 Hystidines


References